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Disc1
| 1 | Two Killers | Alexandre Desplat | 0:37 |
| 2 | The Sisters Brothers | Alexandre Desplat | 3:19 |
| 3 | Duplicity | Alexandre Desplat | 2:26 |
| 4 | To Jacksonville | Alexandre Desplat | 3:26 |
| 5 | Morri's Tricks | Alexandre Desplat | 2:07 |
| 6 | Two Brothers, Two Friends | Alexandre Desplat | 2:23 |
| 7 | To San Francisco | Alexandre Desplat | 3:19 |
| 8 | The Toothbrush | Alexandre Desplat | 2:49 |
| 9 | To Mayfield | Alexandre Desplat | 2:41 |
| 10 | Eli & Tub | Alexandre Desplat | 1:17 |
| 11 | Gun Fight | Alexandre Desplat | 3:43 |
| 12 | Myrtle Creek | Alexandre Desplat | 2:49 |
| 13 | Building the Dam | Alexandre Desplat | 2:16 |
| 14 | Gold! | Alexandre Desplat | 2:44 |
| 15 | Hunted | Alexandre Desplat | 1:52 |
| 16 | Folsom Lake | Alexandre Desplat | 2:55 |
| 17 | To Kill a Commodore | Alexandre Desplat | 2:15 |
| 18 | Dying by the River | Alexandre Desplat | 2:56 |
| 19 | Two Guns, One Hand | Alexandre Desplat | 2:08 |
| 20 | At Home With Mum | Alexandre Desplat | 5:09 |
| 21 | The Sisters Brothers (extended) | Alexandre Desplat | 7:41 |