| 1 | Mule Skinner Blues | Bill Monroe & His Bluegrass Boys | 2:45 |
| 2 | No Letter in the Mail | Bill Monroe & His Bluegrass Boys | 2:42 |
| 3 | Cryin' Holy Unto My Lord | Bill Monroe & His Bluegrass Boys | 2:29 |
| 4 | Six White Horses | Bill Monroe & His Bluegrass Boys | 3:02 |
| 5 | Dog House Blues | Bill Monroe & His Bluegrass Boys | 3:08 |
| 6 | I Wonder If You Feel the Way I Do | Bill Monroe & His Bluegrass Boys | 2:51 |
| 7 | Katy Hill | Bill Monroe & His Bluegrass Boys | 2:39 |
| 8 | Tennessee Blues | Bill Monroe & His Bluegrass Boys | 2:51 |
| 9 | Shake My Mother's Hand for Me | Bill Monroe & His Bluegrass Boys | 3:06 |
| 10 | Were You There? | Bill Monroe & His Bluegrass Boys | 2:47 |
| 11 | Blue Yodel No. 7 | Bill Monroe & His Bluegrass Boys | 2:53 |
| 12 | The Coupon Song | Bill Monroe & His Bluegrass Boys | 2:56 |
| 13 | Orange Blossom Special | Bill Monroe & His Bluegrass Boys | 2:33 |
| 14 | Honky Tonk Swing | Bill Monroe & His Bluegrass Boys | 2:31 |
| 15 | In the Pines | Bill Monroe & His Bluegrass Boys | 3:24 |
| 16 | Back Up and Push | Bill Monroe & His Bluegrass Boys | 2:48 |
| 17 | The Great Speckled Bird | Charlie Monroe's Boys | 2:12 |
| 18 | No Home, No Place to Pillow My Head | Charlie Monroe's Boys | 2:07 |
| 19 | New River Train | The Monroe Brothers | 3:40 |
| 20 | Once I Had a Darling Mother | Charlie Monroe's Boys | 3:20 |
| 21 | On the Banks of the Ohio | The Monroe Brothers | 3:31 |
| 22 | Weeping Willow Tree | The Monroe Brothers | 3:17 |
| 23 | Just a Song of Old Kentucky | The Monroe Brothers | 2:21 |
| 24 | Don't Forget Me | The Monroe Brothers | 2:40 |
| 25 | I Am Thinking Tonight of the Old Folks | The Monroe Brothers | 2:28 |