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Disc1
| 1 | Don't Stop Believin' | Glee Cast | 3:50 |
| 2 | Rehab | Glee Cast | 3:24 |
| 3 | Take a Bow | Glee Cast | 3:35 |
| 4 | Mercy | Glee Cast | 3:27 |
| 5 | Somebody to Love | Glee Cast | 4:42 |
| 6 | Halo / Walking on Sunshine | Glee Cast | 2:05 |
| 7 | Keep Holding On | Glee Cast | 4:01 |
| 8 | Sweet Caroline | Glee Cast | 1:57 |
| 9 | Defying Gravity | Glee Cast | 2:21 |
| 10 | Don't Stand So Close to Me / Young Girl | Glee Cast | 2:26 |
| 11 | True Colors | Glee Cast | 3:33 |
| 12 | My Life Would Suck Without You | Glee Cast | 3:31 |
| 13 | Gives You Hell | Glee Cast | 3:26 |
| 14 | Like a Prayer | Glee Cast | 5:15 |
| 15 | Total Eclipse of the Heart | Glee Cast | 4:25 |
| 16 | Jessie's Girl | Glee Cast | 3:14 |
| 17 | I Dreamed a Dream | Glee Cast | 3:08 |
| 18 | Poker Face | Glee Cast | 3:39 |
| 19 | Over the Rainbow | Glee Cast | 2:32 |
Karaoke Tracks
| 1 | Take a Bow (karaoke version) | Glee Cast | 3:37 |
| 2 | Mercy (karaoke version) | Glee Cast | 3:26 |
| 3 | Jessie's Girl (karaoke version) | Glee Cast | 3:15 |
| 4 | Total Eclipse of the Heart (karaoke version) | Glee Cast | 4:27 |
| 5 | Defying Gravity (karaoke version) | Glee Cast | 2:22 |
| 6 | Gives You Hell (karaoke version) | Glee Cast | 3:27 |